CoraLite®594-conjugated Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10427

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-10427 targets Calnexin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calnexin fusion protein Ag0697 相同性解析による交差性が予測される生物種
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003552
Gene symbol CANX
Gene ID (NCBI) 821
RRIDAB_2919733
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
IF protocol for CL594 Calnexin antibody CL594-10427Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Oncol

Small extracellular vesicles deliver TGF-β1 and promote adriamycin resistance in breast cancer cells.

Authors - Chunli Tan