Calnexin Monoclonal antibody
Calnexin Monoclonal Antibody for IF, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (2)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2A2C6
Cat no : 66903-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF detected in | HeLa cells, HepG2 cells |
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
66903-1-Ig targets Calnexin in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Calnexin fusion protein Ag25535 |
| Full Name | calnexin |
| Calculated molecular weight | 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC003552 |
| Gene symbol | CANX |
| Gene ID (NCBI) | 821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calnexin antibody 66903-1-Ig | Download protocol |
| IF protocol for Calnexin antibody 66903-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chin Med J (Engl) Macrophage exosomes transfer angiotensin II type 1 receptor to lung fibroblasts mediating bleomycin-induced pulmonary fibrosis. | ||
Biochim Biophys Acta Mol Cell Res IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells. | ||
Front Microbiol ANKS4B Restricts Replication of Zika Virus by Downregulating the Autophagy. | ||
Front Oncol Osteosarcoma Cell-Derived Small Extracellular Vesicles Enhance Osteoclastogenesis and Bone Resorption Through Transferring MicroRNA-19a-3p. | ||
Front Cell Dev Biol Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter. | ||
PLoS Pathog Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection. |
