Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, A431 cells, HEK-293 cells, mouse liver tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 35 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19374 Product name: Recombinant human CYP7B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 96-403 aa of BC136574 Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 7, subfamily B, polypeptide 1 |
| Calculated molecular weight | 506 aa, 58 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC136574 |
| Gene Symbol | CYP7B1 |
| Gene ID (NCBI) | 9420 |
| RRID | AB_2879780 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75881 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| IHC protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| WB protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress | ||
Cell Metab Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis | ||
Nat Microbiol Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen | ||
Acta Pharm Sin B A critical role for Phocaeicola vulgatus in negatively impacting metformin response in diabetes | ||
Adv Sci (Weinh) Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury | ||
Phytomedicine Celastrol ameliorates metabolic dysfunction associated steatohepatitis by regulating the CYP7B1-mediated alternative bile acid synthetic pathway |