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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CYP4F11-Specific Polyclonal antibody

CYP4F11-Specific Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20012-1-AP

Synonyms

CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11

Validation Data Gallery

Applications:
  • IHC staining of human hepatocirrhosis using 20012-1-AP

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 20012-1-AP

    IHC staining of human hepatocirrhosis using 20012-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20012-1-AP

    IF Staining of HepG2 using 20012-1-AP

    Immunofluorescent analysis of HepG2 cells, using CYP4F11 antibody 20012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20012-1-AP targets CYP4F11-Specific in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 11
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_001128932
Gene symbol CYP4F11
Gene ID (NCBI) 57834
RRIDAB_10665946
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.

Protocols

Product Specific Protocols
IHC protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
IF protocol for CYP4F11-Specific antibody 20012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse

Int Immunopharmacol

Regulation of cytochrome P450 4F11 expression by liver X receptor alpha.

Authors - Tao Zhang
View Article