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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B10H2

Cat no : 67419-1-Ig

Synonyms

CYP2W1, CYPIIW1, Cytochrome P450 2W1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 67419-1-Ig

    WB analysis of HEK-293 using 67419-1-Ig

    WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

  • WB analysis using 67419-1-Ig

    WB analysis using 67419-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Sample-dependent, check data in validation data gallery

Product Information

67419-1-Ig targets CYP2W1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP2W1 fusion protein Ag29982 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 2, subfamily W, polypeptide 1
Calculated molecular weight 54 kDa
Observed molecular weight 45-54 kDa
GenBank accession numberBC025761
Gene symbol CYP2W1
Gene ID (NCBI) 54905
RRIDAB_2882659
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

Protocols

Product Specific Protocols
WB protocol for CYP2W1 antibody 67419-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols