Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 9 publications below |
| IF | See 3 publications below |
Product Information
21555-1-AP targets CYP26B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15368 Product name: Recombinant human CYP26B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 104-300 aa of BC069443 Sequence: KILMGEHHLVSTEWPRSTRMLLGPNTVSNSIGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRRGIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIFAAYAT 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 26, subfamily B, polypeptide 1 |
| Calculated molecular weight | 512 aa, 58 kDa |
| Observed molecular weight | 49-58 kDa |
| GenBank accession number | BC069443 |
| Gene Symbol | CYP26B1 |
| Gene ID (NCBI) | 56603 |
| RRID | AB_10733471 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| IHC protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| IP protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| WB protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.
| ||
Brain Struct Funct Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1. | ||
J Neurosci The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map. | ||
J Neurosci Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia. | ||
Front Mol Neurosci Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction. | ||
