CoraLite® Plus 488-conjugated CUL2 Monoclonal antibody
CUL2 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A6G9
Cat no : CL488-67175
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67175 targets CUL2 in IF applications and shows reactivity with Human, Rat samples.
| Tested Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CUL2 fusion protein Ag28654 相同性解析による交差性が予測される生物種 |
| Full Name | cullin 2 |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 85-90 kDa |
| GenBank accession number | BC009591 |
| Gene symbol | CUL2 |
| Gene ID (NCBI) | 8453 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CUL2 antibody CL488-67175 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
