Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12921-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12921-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 12921-1-AP
IP result of anti-CUGBP2 (IP:12921-1-AP, 3ug; Detection:12921-1-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.
IHC staining of human breast cancer using 12921-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12921-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12921-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12921-1-AP (CUGBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CUGBP2 Polyclonal antibody

CUGBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12921-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse thymus tissue, K-562 cells, MCF-7 cells
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

12921-1-AP targets CUGBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3594 Product name: Recombinant human CUGBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 175-521 aa of BC036391 Sequence: STRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGTINTPRSKRLLLPKDNNGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY 相同性解析による交差性が予測される生物種
Full Name	CUG triplet repeat, RNA binding protein 2
Calculated molecular weight	56 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC036391
Gene Symbol	CUGBP2
Gene ID (NCBI)	10659
RRID	AB_2230041
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95319
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.

Protocols

Product Specific Protocols
IF protocol for CUGBP2 antibody 12921-1-AP	Download protocol
IHC protocol for CUGBP2 antibody 12921-1-AP	Download protocol
IP protocol for CUGBP2 antibody 12921-1-AP	Download protocol
WB protocol for CUGBP2 antibody 12921-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Aberrant Splicing Signatures Underpin Oligodendrocyte Damage in ALS and Neuron Loss in FTD. Authors - Chen Du KD Validated View Article
human	WB,IF	Pharmacol Res Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway Authors - Ying Zhang View Article
human	IHC	Oncogenesis Investigation of lncRNA expression in newly diagnosed multiple myeloma reveals a LINC01432-CELF2 axis as an inhibitor of apoptosis. Authors - Richa Mishra View Article
human	WB	Oncotarget Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells. Authors - Bo Li View Article
human	WB	Exp Biol Med (Maywood) Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect. Authors - Liuqing Ge View Article
mouse	IHC	Brain Res Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition. Authors - Otsuka Noboru N View Article

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Validation Data Gallery

WB analysis using 12921-1-AP

WB analysis of mouse thymus using 12921-1-AP

WB analysis of K-562 using 12921-1-AP

IP experiment of mouse skeletal muscle using 12921-1-AP

IHC staining of human breast cancer using 12921-1-AP

IHC staining of human breast cancer using 12921-1-AP

IF Staining of HepG2 using 12921-1-AP

CUGBP2 Polyclonal antibody

CUGBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aberrant Splicing Signatures Underpin Oligodendrocyte Damage in ALS and Neuron Loss in FTD.

Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway

Investigation of lncRNA expression in newly diagnosed multiple myeloma reveals a LINC01432-CELF2 axis as an inhibitor of apoptosis.

Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.

Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.

Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.

Able^™

Validation Data Gallery

WB analysis using 12921-1-AP

WB analysis of mouse thymus using 12921-1-AP

WB analysis of K-562 using 12921-1-AP

IP experiment of mouse skeletal muscle using 12921-1-AP

IHC staining of human breast cancer using 12921-1-AP

IHC staining of human breast cancer using 12921-1-AP

IF Staining of HepG2 using 12921-1-AP

CUGBP2 Polyclonal antibody

CUGBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aberrant Splicing Signatures Underpin Oligodendrocyte Damage in ALS and Neuron Loss in FTD.

Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway

Investigation of lncRNA expression in newly diagnosed multiple myeloma reveals a LINC01432-CELF2 axis as an inhibitor of apoptosis.

Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.

Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.

Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.

Able™

Able^™