Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15914-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15914-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15914-1-AP
IP result of anti-CTP synthase (IP:15914-1-AP, 4ug; Detection:15914-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of human liver cancer using 15914-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15914-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15914-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15914-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15914-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTP synthase antibody (15914-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 15914-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTP synthase Polyclonal antibody (15914-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15914-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CTPS, CTPS 1, CTPS1, EC:6.3.4.2, UTP--ammonia ligase 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 2 publications below
IF	See 12 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

15914-1-AP targets CTP synthase in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, zebrafish, mosquito
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8707 Product name: Recombinant human CTPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-591 aa of BC009408 Sequence: ICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRIGSSSPDSEITELKFPSINHD 相同性解析による交差性が予測される生物種
Full Name	CTP synthase
Calculated molecular weight	591 aa, 67 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC009408
Gene Symbol	CTPS
Gene ID (NCBI)	1503
RRID	AB_2086513
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17812
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

Protocols

Product Specific Protocols
IF protocol for CTP synthase antibody 15914-1-AP	Download protocol
IHC protocol for CTP synthase antibody 15914-1-AP	Download protocol
IP protocol for CTP synthase antibody 15914-1-AP	Download protocol
WB protocol for CTP synthase antibody 15914-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells. Authors - Zhe Sun View Article
human	IF	FEBS J Inter-filament interaction between IMPDH and CTPS cytoophidia. Authors - Chia-Chun Chang View Article
human	WB,CoIP	J Cell Physiol Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization. Authors - Ke Ma View Article
human		Front Immunol Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation. Authors - Otavio Cabral-Marques View Article
human	WB	Oncotarget Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics. Authors - Hong Qu View Article
human	IF	J Cell Sci Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway. Authors - S John Calise View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15914-1-AP

WB analysis of HeLa using 15914-1-AP

IP experiment of HeLa using 15914-1-AP

IHC staining of human liver cancer using 15914-1-AP

IHC staining of human liver cancer using 15914-1-AP

IHC staining of human liver cancer using 15914-1-AP

IHC staining of human liver cancer using 15914-1-AP

IF Staining of HepG2 using 15914-1-AP

FC experiment of HepG2 using 15914-1-AP

CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.

Inter-filament interaction between IMPDH and CTPS cytoophidia.

Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization.

Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation.

Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics.

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.