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- KD/KO Validated
CTP synthase Polyclonal antibody
CTP synthase Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15914-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 11 publications below |
| CoIP | See 1 publications below |
Product Information
15914-1-AP targets CTP synthase in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mosquito, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CTP synthase fusion protein Ag8707 相同性解析による交差性が予測される生物種 |
| Full Name | CTP synthase |
| Calculated molecular weight | 591 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC009408 |
| Gene symbol | CTPS |
| Gene ID (NCBI) | 1503 |
| RRID | AB_2086513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTP synthase antibody 15914-1-AP | Download protocol |
| IHC protocol for CTP synthase antibody 15914-1-AP | Download protocol |
| IF protocol for CTP synthase antibody 15914-1-AP | Download protocol |
| IP protocol for CTP synthase antibody 15914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells. | ||
J Cell Physiol Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization. | ||
Front Immunol Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation. | ||
Oncotarget Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics. | ||
J Cell Sci Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway. |
