Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, Jurkat cells, mouse lung tissue |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | A375 cells, HepG2 cells |
| Positive FC (Intra) detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 20 publications below |
| IHC | See 14 publications below |
| IF | See 9 publications below |
| CoIP | See 1 publications below |
Product Information
16534-1-AP targets CTHRC1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9812 Product name: Recombinant human CTHRC1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-243 aa of BC014245 Sequence: MRPQGPAASPQRLRGLLLLLLLQLPAPSSASEIPKGKQKAQLRQREVVDLYNGMCLQGPAGVPGRDGSPGANGIPGTPGIPGRDGFKGEKGECLRESFEESWTPNYKQCSWSSLNYGIDLGKIAECTFTKMRSNSALRVLFSGSLRLKCRNACCQRWYFTFNGAECSGPLPIEAIIYLDQGSPEMNSTINIHRTSSVEGLCEGIGAGLVDVAIWVGTCSDYPKGDASTGWNSVSRIIIEELPK 相同性解析による交差性が予測される生物種 |
| Full Name | collagen triple helix repeat containing 1 |
| Calculated molecular weight | 243 aa, 26 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC014245 |
| Gene Symbol | CTHRC1 |
| Gene ID (NCBI) | 115908 |
| RRID | AB_2087511 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CTHRC1 antibody 16534-1-AP | Download protocol |
| IP protocol for CTHRC1 antibody 16534-1-AP | Download protocol |
| WB protocol for CTHRC1 antibody 16534-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer. | ||
J Transl Med Single-cell and spatial transcriptome profiling reveal CTHRC1+ fibroblasts promote EMT through WNT5A signaling in colorectal cancer | ||
Respir Res Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1. | ||
J Cell Sci miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.
| ||
Inflammation Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro
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