Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
10952-1-AP targets CTDSP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1410 Product name: Recombinant human CTDSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC012977 Sequence: MDSSAVITQISKEEARGPLRGKGDQKSAASQKPRSRGILHSLFCCVCRDDGEALPAHSGAPLLVEENGAIPKQTPVQYLLPEAKAQDSDKICVVIDLDETLVHSSFKPVNNADFIIPVEIDGVVHQVYVLKRPHVDEFLQRMGELFECVLFTASLAKYADPVADLLDKWGAFRARLFRESCVFHRGNYVKDLSRLGRDLRRVLILDNSPASYVFHPDNAVPVASWFDNMSDTELHDLLPFFEQLSRVDDVYSVLRQPRPGS 相同性解析による交差性が予測される生物種 |
| Full Name | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 29-33 kDa |
| GenBank accession number | BC012977 |
| Gene Symbol | CTDSP1 |
| Gene ID (NCBI) | 58190 |
| RRID | AB_2087073 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTDSP1 also named as NIF3, NLIIF, or SCP1, is a 261 amino acid protein, which contains one FCP1 homology domain. CTDSP1 localizes in the nucleus and colocalizes with RNA polymerase II. CTDSP1 is expressed in non-neuronal tissues. It is highly expressed in skeletal muscle, spleen, lung and placenta. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTDSP1 antibody 10952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Phosphatase activity of Small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).
| ||
Iran J Basic Med Sci MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma | ||
Cell Mol Neurobiol Elabela, a Novel Peptide, Exerts Neuroprotective Effects Against Ischemic Stroke Through the APJ/miR-124-3p/CTDSP1/AKT Pathway |
