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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NY-ESO-1 Polyclonal antibody

NY-ESO-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19521-1-AP

Synonyms

Cancer/testis antigen 1, cancer/testis antigen 1A, Cancer/testis antigen 6.1, CT6.1, CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, L antigen family member 2, LAGE 2, LAGE2, LAGE2A, LAGE2B, NY-ESO-1

Validation Data Gallery

Applications:
  • WB analysis of human testis using 19521-1-AP

    WB analysis of human testis using 19521-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-1080 using 19521-1-AP

    WB analysis of HT-1080 using 19521-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis using 19521-1-AP

    WB analysis using 19521-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19521-1-AP

    IP experiment of HepG2 using 19521-1-AP

    IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • IHC staining of mouse testis using 19521-1-AP

    IHC staining of mouse testis using 19521-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 19521-1-AP

    IHC staining of mouse testis using 19521-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 19521-1-AP

    IF Staining of HepG2 using 19521-1-AP

    Immunofluorescent analysis of HepG2 cells, using CTAG1A antibody 19521-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman testis tissue, HT-1080 cells, NCI-H1299 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

19521-1-AP targets NY-ESO-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cancer/testis antigen 1A
Calculated molecular weight 18 kDa
Observed molecular weight 16-25 kDa
GenBank accession numberNM_139250
Gene symbol CTAG1A
Gene ID (NCBI) 246100
RRIDAB_10638144
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.

Protocols

Product Specific Protocols
WB protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IHC protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IF protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IP protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Immunol Res

CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance

Authors - Liyan Lao
View Article
humanWB

Nat Cancer

The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer.

Authors - Jiang Liu
View Article