Validation Data Gallery
Tested Applications
| Positive WB detected in | NK-92 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 23 publications below |
| IHC | See 17 publications below |
| IF | See 8 publications below |
Product Information
17762-1-AP targets GM-CSF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12142 Product name: Recombinant human CSF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC108724 Sequence: MWLQSLLLLGTVACSISAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE 相同性解析による交差性が予測される生物種 |
| Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Calculated molecular weight | 144 aa, 16 kDa |
| Observed molecular weight | 15kDa,18 kDa ,22 kDa |
| GenBank accession number | BC108724 |
| Gene Symbol | GM-CSF |
| Gene ID (NCBI) | 1437 |
| RRID | AB_2276718 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04141 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GM-CSF antibody 17762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hematol Oncol FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion | ||
Nat Nanotechnol A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy | ||
Adv Sci (Weinh) Modular Hydrogel Vaccine for Programmable and Coordinate Elicitation of Cancer Immunotherapy | ||
Cancer Lett HIF-1α mediates tumor-nerve interactions through the up-regulation of GM-CSF in pancreatic ductal adenocarcinoma. | ||
J Immunother Cancer Intratumoral oncolytic virus OH2 injection in patients with locally advanced or metastatic sarcoma: a phase 1/2 trial |