Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 22219-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 22219-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 22219-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22219-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 22219-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22219-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22219-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22219-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 22219-1-AP
IP result of anti-CSE1L (IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.
IHC staining of human placenta using 22219-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 22219-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 22219-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 22219-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CSE1L Polyclonal antibody

CSE1L Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22219-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, mouse testis tissue, RAW264.7, MCF-7 cells, Ramos cells, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human placenta tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

22219-1-AP targets CSE1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17556 Product name: Recombinant human CSE1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 627-971 aa of BC109313 Sequence: RITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL 相同性解析による交差性が予測される生物種
Full Name	CSE1 chromosome segregation 1-like (yeast)
Calculated molecular weight	971 aa, 110 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC109313
Gene Symbol	CSE1L
Gene ID (NCBI)	1434
RRID	AB_10950892
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55060
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

Protocols

Product Specific Protocols
IHC protocol for CSE1L antibody 22219-1-AP	Download protocol
IP protocol for CSE1L antibody 22219-1-AP	Download protocol
WB protocol for CSE1L antibody 22219-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk Authors - Pingting Ying View Article
human	WB	Cancers (Basel) GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells. Authors - Chiara Bellio View Article
human	WB,IHC	Sci Rep CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival. Authors - Dong-Dong Cheng KD Validated View Article
human	WB	J Biol Chem RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome. Authors - Jackson B Trotman View Article
human,mouse	WB,IHC,IF	Int J Oncol Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer. Authors - Chun-Chao Chang KD Validated View Article
human	WB	J Cancer CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway. Authors - Xiao Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22219-1-AP

WB analysis using 22219-1-AP

WB analysis using 22219-1-AP

WB analysis of HEK-293 using 22219-1-AP

WB analysis of mouse testis using 22219-1-AP

WB analysis of RAW264.7 using 22219-1-AP

WB analysis of MCF-7 using 22219-1-AP

WB analysis of HeLa using 22219-1-AP

IP experiment of HEK-293 using 22219-1-AP

IHC staining of human placenta using 22219-1-AP

IHC staining of human placenta using 22219-1-AP

IHC staining of mouse testis using 22219-1-AP

IHC staining of mouse testis using 22219-1-AP

CSE1L Polyclonal antibody

CSE1L Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer.

CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway.