CRYBB1 Monoclonal antibody

CRYBB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6F12B12

Cat no : 60273-1-Ig

Synonyms

Beta B1 crystallin, Beta crystallin B1, CATCN3, CRYBB1, crystallin, beta B1


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Tested Applications

Positive WB detected inC6 cells, mouse eye tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60273-1-Ig targets CRYBB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRYBB1 fusion protein Ag4722 相同性解析による交差性が予測される生物種
Full Name crystallin, beta B1
Calculated molecular weight 252 aa, 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC036790
Gene symbol CRYBB1
Gene ID (NCBI) 1414
RRIDAB_2881393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.

Protocols

Product Specific Protocols
WB protocol for CRYBB1 antibody 60273-1-IgDownload protocol
IF protocol for CRYBB1 antibody 60273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols