Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
12220-1-AP targets CREG1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CREG1 fusion protein Ag2901 相同性解析による交差性が予測される生物種 |
| Full Name | cellular repressor of E1A-stimulated genes 1 |
| Calculated molecular weight | 220 aa, 24 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC008628 |
| Gene Symbol | CREG1 |
| Gene ID (NCBI) | 8804 |
| RRID | AB_2084095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CREG1 antibody 12220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. | ||
Int J Mol Sci TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle. | ||
Carcinogenesis PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1 | ||
J Cell Mol Med Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats. | ||
J Biochem CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.
| ||
FASEB J CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice. |