Validation Data Gallery

Filter: AllWBIPIHCIF-PFC (Intra)

WB analysis of Raji using 14348-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14348-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14348-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 14348-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 14348-1-AP
IP result of anti-CRCP (IP:14348-1-AP, 3ug; Detection:14348-1-AP 1:1000) with Raji cells lysate 2800ug.
IHC staining of human testis using 14348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14348-1-AP (CRCP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14348-1-AP (CRCP Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse testis using 14348-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CRCP antibody (14348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of Raji using 14348-1-AP
1x10^6 Raji cells were intracellularly stained with 0.25 ug CRCP Polyclonal antibody (14348-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CRCP Polyclonal antibody

CRCP Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 14348-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

Calcitonin gene-related peptide-receptor component protein, CGRP RCP, CGRP receptor component, CGRPRCP, CGRP-RCP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, HeLa cells, human testis tissue, MCF-7 cells
Positive IP detected in	Raji cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive FC (Intra) detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 5 publications below

Product Information

14348-1-AP targets CRCP in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5689 Product name: Recombinant human CRCP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-115 aa of BC040107 Sequence: MEVKDANSALLSNYETLKYISKTPCRHQSPEIVREFLTALKSHKLTKAEKLQLLNHRPVTAVEIQLMVEESEERLTEEQIEALLHTVTSILPAEPEAEQKKNTNSNVAMDEEDPA 相同性解析による交差性が予測される生物種
Full Name	CGRP receptor component
Calculated molecular weight	17 kDa
Observed molecular weight	20 kDa
GenBank accession number	BC040107
Gene Symbol	CRCP
Gene ID (NCBI)	27297
RRID	AB_10641051
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75575
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CRCP antibody 14348-1-AP	Download protocol
IHC protocol for CRCP antibody 14348-1-AP	Download protocol
IP protocol for CRCP antibody 14348-1-AP	Download protocol
WB protocol for CRCP antibody 14348-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat,mouse	IF	J Neuroinflammation Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain. Authors - Qi An View Article
human	WB	mSphere Genome-scale CRISPR/Cas9 screening reveals the role of PSMD4 in colibactin-mediated cell cycle arrest Authors - Michael W Dougherty View Article
human	WB	J Transl Med CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis. Authors - Rui Shi View Article
rat	WB,IF	CNS Neurosci Ther Calcitonin gene-related peptide induces the histone H3 lysine 9 acetylation in astrocytes associated with neuroinflammation in rats with neuropathic pain. Authors - Chenyan Sun View Article
mouse	IF	Neurogastroenterol Motil Altered gene expression signatures by calcitonin gene-related peptide promoted mast cell activity in the colon of stress-induced visceral hyperalgesia mice. Authors - Hanliang Sun View Article
rat	IF	Cell Biochem Funct CNTs-CaP/chitosan-coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway. Authors - Tingjiao Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Raji using 14348-1-AP

WB analysis of MCF-7 using 14348-1-AP

WB analysis of HeLa using 14348-1-AP

WB analysis of human testis using 14348-1-AP

IP experiment of Raji using 14348-1-AP

IHC staining of human testis using 14348-1-AP

IHC staining of human testis using 14348-1-AP

IF Staining of mouse testis using 14348-1-AP

FC experiment of Raji using 14348-1-AP

CRCP Polyclonal antibody

CRCP Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain.

Genome-scale CRISPR/Cas9 screening reveals the role of PSMD4 in colibactin-mediated cell cycle arrest

CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.

Calcitonin gene-related peptide induces the histone H3 lysine 9 acetylation in astrocytes associated with neuroinflammation in rats with neuropathic pain.

Altered gene expression signatures by calcitonin gene-related peptide promoted mast cell activity in the colon of stress-induced visceral hyperalgesia mice.

CNTs-CaP/chitosan-coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway.