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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRACR2A Monoclonal antibody

CRACR2A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C1F5

Cat no : CL594-66787

Synonyms

CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL594-66787

    IF Staining of HEK-293 using CL594-66787

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 CRACR2A antibody (CL594-66787, Clone: 4C1F5 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66787-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66787 targets CRACR2A in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRACR2A fusion protein Ag7525 相同性解析による交差性が予測される生物種
Full Name EF-hand calcium binding domain 4B
Calculated molecular weight 46 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC004524
Gene symbol EFCAB4B
Gene ID (NCBI) 84766
RRIDAB_2920038
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

Protocols

Product Specific Protocols
IF protocol for CL594 CRACR2A antibody CL594-66787Download protocol
Standard Protocols
Click here to view our Standard Protocols