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  • Featured Product
  • KD/KO Validated

CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10225-1-AP

Synonyms

CRABP II, CRABP2, RBP6

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 10225-1-AP

    WB analysis of HeLa using 10225-1-AP

    WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.

  • WB analysis using 10225-1-AP

    WB analysis using 10225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10225-1-AP

    IP experiment of HeLa using 10225-1-AP

    IP result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.

  • IHC staining of human skin using 10225-1-AP

    IHC staining of human skin using 10225-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 10225-1-AP

    IHC staining of human skin using 10225-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 10225-1-AP

    IF Staining of MCF-7 using 10225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of human skin cancer using 10225-1-AP

    IF Staining of human skin cancer using 10225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 10225-1-AP

    FC experiment of MCF-7 using 10225-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human skin cancer tissue
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10225-1-AP targets CRABP2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309 相同性解析による交差性が予測される生物種
Full Name cellular retinoic acid binding protein 2
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC001109
Gene symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2085455
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
WB protocol for CRABP2 antibody 10225-1-APDownload protocol
IHC protocol for CRABP2 antibody 10225-1-APDownload protocol
IF protocol for CRABP2 antibody 10225-1-APDownload protocol
IP protocol for CRABP2 antibody 10225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

A single progenitor population switches behavior to maintain and repair esophageal epithelium.

Authors - David P Doupé
View Article
humanWB

Mol Cell

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Authors - Ai Kaiho-Soma
View Article
humanWB,IF

Neuron

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

Authors - Alana I Mendelsohn
View Article
mouseIF

Nat Commun

The developing mouse coronal suture at single-cell resolution.

Authors - D'Juan T Farmer
View Article
mouseIF

Dev Cell

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

Authors - John DeSisto
View Article
humanIF

Stem Cell Reports

BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.

Authors - Kai Kretzschmar
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse embryonic section
FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse brain tissue