Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12211-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12211-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 12211-1-AP
IP result of anti-CPOX (IP:12211-1-AP, 4ug; Detection:12211-1-AP 1:500) with HepG2 cells lysate 2400ug.
IHC staining of human liver cancer using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver cancer using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12211-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CPOX Polyclonal antibody

CPOX Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12211-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

12211-1-AP targets CPOX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2858 Product name: Recombinant human CPOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC023551 Sequence: MALQLGRLSSGPCWLVARGGCGGPRAWSQCGGGGLRAWSQRSAAGRVCRPPGPAGTEQSRGLGHGSTSRGGPWVGTGLAAALAGLVGLATAAFGHVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGNKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEA 相同性解析による交差性が予測される生物種
Full Name	coproporphyrinogen oxidase
Calculated molecular weight	454 aa, 50 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC023551
Gene Symbol	CPOX
Gene ID (NCBI)	1371
RRID	AB_2084233
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36551
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

Protocols

Product Specific Protocols
IHC protocol for CPOX antibody 12211-1-AP	Download protocol
IP protocol for CPOX antibody 12211-1-AP	Download protocol
WB protocol for CPOX antibody 12211-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	J Cell Biol Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination. Authors - Yumiko Oshima View Article
human	IHC	Neuro Oncol Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence. Authors - Takahashi Kenkichi K View Article
human,mouse	WB	Biochem Pharmacol Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis. Authors - Katalin Módis KD Validated View Article
human	IHC	Front Med (Lausanne) Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas. Authors - Mario Mischkulnig View Article
human	IHC	Photodiagnosis Photodyn Ther Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis. Authors - Kumi Ogihara View Article
human	IHC	Photodiagnosis Photodyn Ther Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer. Authors - Hiroki Kurumi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12211-1-AP

WB analysis of K-562 using 12211-1-AP

IP experiment of HepG2 using 12211-1-AP

IHC staining of human liver cancer using 12211-1-AP

IHC staining of human liver cancer using 12211-1-AP

IHC staining of human liver using 12211-1-AP

IHC staining of human liver using 12211-1-AP

IHC staining of human liver cancer using 12211-1-AP

IHC staining of human liver cancer using 12211-1-AP

CPOX Polyclonal antibody

CPOX Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.

Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence.

Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.

Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.

Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.

Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer.