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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CPE Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CPE Polyclonal antibody (13710-1-AP)

    WB analysis using 13710-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13710-1-AP (CPE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IP experiment of mouse brain using 13710-1-AP

    IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of mouse brain using 13710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of mouse brain using 13710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of rat brain using 13710-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPE Polyclonal antibody (13710-1-AP)

    IF Staining of mouse pancreas using 13710-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPE antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPE Polyclonal antibody (13710-1-AP)

    IF Staining of mouse pancreas using 13710-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPE antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CPE Polyclonal antibody (13710-1-AP)

    IF Staining of mouse brain using 13710-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IF Staining of HepG2 using 13710-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

CPE Polyclonal antibody

CPE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 13710-1-AP
Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, PC-12 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pituitary tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13710-1-AP targets CPE in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4647

Product name: Recombinant human CPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-476 aa of BC033866

Sequence: FLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF

相同性解析による交差性が予測される生物種
Full Name carboxypeptidase E
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC033866
Gene Symbol Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_2083913
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP16870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPE(Carboxypeptidase E) is also named as CPH. It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Protocols

Product Specific Protocols
IF protocol for CPE antibody 13710-1-APDownload protocol
IHC protocol for CPE antibody 13710-1-APDownload protocol
IP protocol for CPE antibody 13710-1-APDownload protocol
WB protocol for CPE antibody 13710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell

Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2.

Authors - Xinyan Li
View Article
ratWB

Cell Metab

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Authors - Guo-Fang Zhang
View Article
mouseWB,IHC,IF

Transl Neurodegener

Agomirs upregulating carboxypeptidase E expression rescue hippocampal neurogenesis and memory deficits in Alzheimer's disease

Authors - Dongfang Jiang
  • KD Validated
View Article
humanIF

Cancer Lett

CPE/HYOU1 axis-activated Hippo-YAP signaling pathway suppresses PANoptosis to facilitate osteosarcoma progression.

Authors - Tong Zhu
  • KD Validated
View Article
mouseWB

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
View Article
ratIF

J Cell Biol

Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules

Authors - Anup Parchure
View Article
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