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  • KD/KO Validated

COX7A2L Polyclonal antibody

COX7A2L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11416-1-AP

Synonyms

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81


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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11416-1-AP targets COX7A2L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX7A2L fusion protein Ag1980 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit VIIa polypeptide 2 like
Calculated molecular weight 114 aa, 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC007095
Gene symbol COX7A2L
Gene ID (NCBI) 9167
RRIDAB_2245402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Protocols

Product Specific Protocols
WB protocol for COX7A2L antibody 11416-1-APDownload protocol
IHC protocol for COX7A2L antibody 11416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

Supercomplex assembly determines electron flux in the mitochondrial electron transport chain.

Authors - Esther Lapuente-Brun
humanWB

EMBO J

Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.

Authors - Teresa Lobo-Jarne
humanWB

Cell Rep

Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.

Authors - Teresa Lobo-Jarne
  • KO Validated

Free Radic Biol Med

Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.

Authors - I Ramírez-Camacho
humanWB

Cell Rep

COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.

Authors - Rafael Pérez-Pérez
humanWB,IHC,IF

J Pathol

Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.

Authors - Aichler Michaela M
  • KD Validated