Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11463-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11463-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.
WB analysis using 11463-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11463-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11463-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11463-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11463-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment using 11463-1-AP
IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate.
IP experiment of HepG2 using 11463-1-AP
IP result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug.
IHC staining of human ovary tumor using 11463-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11463-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 11463-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11463-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

COX IV 2, COX IV-2, COX4, COX4 2, COX4B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, A375 cells, human brain tissue, MCF-7 cells, mouse heart tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 15 publications below
IHC	See 4 publications below
IF	See 4 publications below

Product Information

11463-1-AP targets COX4I2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1987 Product name: Recombinant human COX4I2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC057779 Sequence: MLPRAAWSLVLRKGGGGRRGMHSSEGTTRGGGKMSPYTNCYAQRYYPMPEEPFCTELNAEEQALKEKEKGSWTQLTHAEKVALYRLQFNETFAEMNRRSNEWKTVMGCVFFFIGFAALVIWWQRVYVFPPKPITLTDERKAQQLQRMLDMKVNPVQGLASRWDYEKKQWKK 相同性解析による交差性が予測される生物種
Full Name	cytochrome c oxidase subunit IV isoform 2 (lung)
Calculated molecular weight	171 aa, 20 kDa
Observed molecular weight	17-18 kDa
GenBank accession number	BC057779
Gene Symbol	COX4I2
Gene ID (NCBI)	84701
RRID	AB_2085287
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KJ9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

Protocols

Product Specific Protocols
IF protocol for COX4I2 antibody 11463-1-AP	Download protocol
IHC protocol for COX4I2 antibody 11463-1-AP	Download protocol
IP protocol for COX4I2 antibody 11463-1-AP	Download protocol
WB protocol for COX4I2 antibody 11463-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Discov Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma Authors - Megan E Bischoff View Article
mouse	IHC	Nat Commun Hif1α-dependent mitochondrial acute O2 sensing and signaling to myocyte Ca2+ channels mediate arterial hypoxic vasodilation Authors - Alejandro Moreno-Domínguez View Article
		Sci Adv Putative PINK1/Parkin activators lower the threshold for mitophagy by sensitizing cells to mitochondrial stress Authors - William M Rosencrans View Article
rat	WB	Basic Res Cardiol Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat Authors - Ling Li View Article
human	WB	Antioxidants (Basel) Cytochrome c Oxidase Subunit COX4-1 Reprograms Erastin-Induced Cell Death from Ferroptosis to Apoptosis: A Transmitochondrial Study. Authors - Claudia R. Oliva View Article
human	WB	Free Radic Biol Med Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma. Authors - Md Yousuf Ali View Article

Validation Data Gallery

WB analysis using 11463-1-AP

WB analysis using 11463-1-AP

WB analysis using 11463-1-AP

WB analysis of A375 using 11463-1-AP

WB analysis of MCF-7 using 11463-1-AP

WB analysis of HepG2 using 11463-1-AP

WB analysis of human brain using 11463-1-AP

IP experiment using 11463-1-AP

IP experiment of HepG2 using 11463-1-AP

IHC staining of human ovary tumor using 11463-1-AP

IF Staining of HeLa using 11463-1-AP

IF Staining of HepG2 using 11463-1-AP

COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma

Hif1α-dependent mitochondrial acute O2 sensing and signaling to myocyte Ca2+ channels mediate arterial hypoxic vasodilation

Putative PINK1/Parkin activators lower the threshold for mitophagy by sensitizing cells to mitochondrial stress

Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat

Cytochrome c Oxidase Subunit COX4-1 Reprograms Erastin-Induced Cell Death from Ferroptosis to Apoptosis: A Transmitochondrial Study.

Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.

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