Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11463-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.
WB analysis of MCF-7 using 11463-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11463-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11463-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11463-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11463-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11463-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11463-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11463-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11463-1-AP
IP result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug.
IP experiment using 11463-1-AP
IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate.
IHC staining of human ovary tumor using 11463-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11463-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 11463-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11463-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

COX4L2, COX4B, COX4 2, COX4, COX IV-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 4 publications below
IF	See 4 publications below

Product Information

11463-1-AP targets COX4I2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1987 Product name: Recombinant human COX4I2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC057779 Sequence: MLPRAAWSLVLRKGGGGRRGMHSSEGTTRGGGKMSPYTNCYAQRYYPMPEEPFCTELNAEEQALKEKEKGSWTQLTHAEKVALYRLQFNETFAEMNRRSNEWKTVMGCVFFFIGFAALVIWWQRVYVFPPKPITLTDERKAQQLQRMLDMKVNPVQGLASRWDYEKKQWKK 相同性解析による交差性が予測される生物種
Full Name	cytochrome c oxidase subunit IV isoform 2 (lung)
Calculated molecular weight	171 aa, 20 kDa
Observed molecular weight	17-18 kDa
GenBank accession number	BC057779
Gene Symbol	COX4I2
Gene ID (NCBI)	84701
RRID	AB_2085287
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KJ9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

Protocols

Product Specific Protocols
IF protocol for COX4I2 antibody 11463-1-AP	Download protocol
IHC protocol for COX4I2 antibody 11463-1-AP	Download protocol
IP protocol for COX4I2 antibody 11463-1-AP	Download protocol
WB protocol for COX4I2 antibody 11463-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Rep Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia. Authors - Arpita Chowdhury View Article
human	WB	Oncotarget Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas. Authors - Claudia R Oliva KD Validated View Article
mouse	IF	Sci Signal Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors. Authors - Alejandro Moreno-Domínguez KO Validated View Article
human	WB	Free Radic Biol Med Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma. Authors - Md Yousuf Ali View Article
human	WB	Bioconjug Chem GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery. Authors - Theodoros Karampelas View Article
human	WB	Free Radic Biol Med Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress. Authors - Schneider Lonnie L View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11463-1-AP

WB analysis of MCF-7 using 11463-1-AP

WB analysis of HepG2 using 11463-1-AP

WB analysis of A375 using 11463-1-AP

WB analysis of MCF-7 using 11463-1-AP

WB analysis of human brain using 11463-1-AP

WB analysis of human brain using 11463-1-AP

WB analysis of human heart using 11463-1-AP

WB analysis of human lung using 11463-1-AP

IP experiment of HepG2 using 11463-1-AP

IP experiment using 11463-1-AP

IHC staining of human ovary tumor using 11463-1-AP

IF Staining of HeLa using 11463-1-AP

IF Staining of HepG2 using 11463-1-AP

COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.

Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.

Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.

Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.

GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.

Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.