Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HepG2 cells, COLO 320 cells, mouse kidney tissue, mouse spleen tissue, rat kidney tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
17251-1-AP targets COL8A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10607 Product name: Recombinant human COL8A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 396-744 aa of BC013581 Sequence: GIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type VIII, alpha 1 |
| Calculated molecular weight | 744 aa, 73 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC013581 |
| Gene Symbol | COL8A1 |
| Gene ID (NCBI) | 1295 |
| RRID | AB_10696173 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27658 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| IP protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| WB protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Bioeng Biotechnol Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts. | ||
Front Cell Dev Biol Platelet-Derived Extracellular Vesicles Increase Col8a1 Secretion and Vascular Stiffness in Intimal Injury.
| ||
FASEB J Silencing of METTL3 attenuates cardiac fibrosis induced by myocardial infarction via inhibiting the activation of cardiac fibroblasts. | ||
Biomed Pharmacother Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. | ||
Dis Model Mech Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis. |
