Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of Raji using 11135-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11135-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11135-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11135-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11135-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 11135-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11135-1-AP
IP result of anti-CNOT3 (IP:11135-1-AP, 4ug; Detection:11135-1-AP 1:1000) with HeLa cells lysate 3200ug.
IF Staining of HeLa using 11135-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNOT3 antibody (11135-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CNOT3 Polyclonal antibody

CNOT3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 11135-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

NOT3, LENG2, KIAA0691, CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 12 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

11135-1-AP targets CNOT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1587 Product name: Recombinant human CNOT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-753 aa of BC016474 Sequence: NASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ 相同性解析による交差性が予測される生物種
Full Name	CCR4-NOT transcription complex, subunit 3
Calculated molecular weight	120 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC016474
Gene Symbol	CNOT3
Gene ID (NCBI)	4849
RRID	AB_2229682
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75175
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].

Protocols

Product Specific Protocols
IF protocol for CNOT3 antibody 11135-1-AP	Download protocol
IP protocol for CNOT3 antibody 11135-1-AP	Download protocol
WB protocol for CNOT3 antibody 11135-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Authors - G Gregory Neely KO Validated View Article
human	WB	Nucleic Acids Res Alternative splicing of CNOT7 diversifies CCR4-NOT functions. Authors - Clément Chapat View Article
human	WB	RNA An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21. Authors - Ewan K S McRae View Article
human	WB	Apoptosis PMID: 31177396 Authors - Lin Jing KD Validated View Article
human	WB	Nat Cancer ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1. Authors - Tong Zhang View Article
mouse	WB,IF	bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance Authors - Qing Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Raji using 11135-1-AP

WB analysis of mouse brain using 11135-1-AP

WB analysis of HEK-293 using 11135-1-AP

WB analysis of K-562 using 11135-1-AP

WB analysis of human brain using 11135-1-AP

WB analysis of HT-1080 using 11135-1-AP

IP experiment of HeLa using 11135-1-AP

IF Staining of HeLa using 11135-1-AP

CNOT3 Polyclonal antibody

CNOT3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.

Alternative splicing of CNOT7 diversifies CCR4-NOT functions.

An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.

PMID: 31177396

ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.

Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance