Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10852-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10852-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10852-1-AP
IP result of anti-CLTA (IP:10852-1-AP, 4ug; Detection:10852-1-AP 1:800) with mouse brain tissue lysate 4000ug.
IHC staining of human breast cancer using 10852-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10852-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10852-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10852-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CLTA antibody (10852-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of HEK-293 using 10852-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CLTA Polyclonal antibody

CLTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10852-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LCA, clathrin, light chain (Lca), Clathrin light chain A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, K-562 cells, Raji cells, C2C12 cells, C6 cells, PC-12 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 12 publications below
IP	See 1 publications below

Product Information

10852-1-AP targets CLTA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1299 Product name: Recombinant human CLTA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC019287 Sequence: MAELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGPQPHGEPPGGPDAVDGVMNGEYYQESNGPTDSYAAISQVDRLQSEPESIRKWREEQMERLEALDANSRKQEAEWKEKAIKELEEWYARQDEQLQKTKANNRAAEEAFVNDIDESSPGTEWERVARLCDFNPKSSKQAKDVSRMRSVLISLKQAPLVH 相同性解析による交差性が予測される生物種
Full Name	clathrin, light chain (Lca)
Calculated molecular weight	27 kDa
Observed molecular weight	35-38 kDa
GenBank accession number	BC019287
Gene Symbol	CLTA
Gene ID (NCBI)	1211
RRID	AB_2083025
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09496
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
IF protocol for CLTA antibody 10852-1-AP	Download protocol
IHC protocol for CLTA antibody 10852-1-AP	Download protocol
IP protocol for CLTA antibody 10852-1-AP	Download protocol
WB protocol for CLTA antibody 10852-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis. Authors - Tomasz J Nawara View Article
human	WB	Proc Natl Acad Sci U S A Clathrin light chains' role in selective endocytosis influences antibody isotype switching. Authors - Shuang Wu KO Validated View Article
human	WB	J Cell Biol Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques. Authors - Gilles Moulay View Article
		Small Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes. Authors - Ji-Qian Zhang View Article
rat	IF	Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy Authors - Ji-Chao Wu View Article
rat	IHC	J Bone Miner Res Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis. Authors - Lacruz Rodrigo S RS View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10852-1-AP

WB analysis of mouse brain using 10852-1-AP

IP experiment of mouse brain using 10852-1-AP

IHC staining of human breast cancer using 10852-1-AP

IHC staining of human breast cancer using 10852-1-AP

IF Staining of HepG2 using 10852-1-AP

IF Staining of HepG2 using 10852-1-AP

IF Staining of HEK-293 using 10852-1-AP

CLTA Polyclonal antibody

CLTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.

Clathrin light chains' role in selective endocytosis influences antibody isotype switching.

Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.

Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.