Chromogranin A Polyclonal antibody
Chromogranin A Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10529-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-12 cells |
| Positive IHC detected in | human pancreas tissue, human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
10529-1-AP targets Chromogranin A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chromogranin A fusion protein Ag0807 相同性解析による交差性が予測される生物種 |
| Full Name | chromogranin A (parathyroid secretory protein 1) |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC006459 |
| Gene symbol | CHGA |
| Gene ID (NCBI) | 1113 |
| RRID | AB_2081122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
| IHC protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
| IF protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance. | ||
Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer | ||
Sci Rep Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line. | ||
Discov Oncol Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma | ||
Dose Response The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury. | ||
Bioengineered A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH balawant (Verified Customer) (07-26-2022) | This Antibody is working great. I have used for colon and colon cancer cell line
|
FH Susmita (Verified Customer) (04-13-2022) | This antibody works excellent.
|
FH James (Verified Customer) (03-31-2021) | Antibody worked well in both recombinant Chromogranin A produced in both HeLa cell lysates and in HEK cells overexpressed with Chromogranin A
|
FH Iram (Verified Customer) (08-14-2020) | Used 1:1000 dilution for immunoblotting. It gives a very clean band.
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