Validation Data Gallery
Tested Applications
| Positive WB detected in | SGC-7901 cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
13402-1-AP targets CHD9 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHD9 fusion protein Ag4245 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain helicase DNA binding protein 9 |
| Calculated molecular weight | 2897 aa, 326 kDa |
| Observed molecular weight | 330 kDa |
| GenBank accession number | BC033770 |
| Gene Symbol | CHD9 |
| Gene ID (NCBI) | 80205 |
| RRID | AB_10640425 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3L8U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IHC protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IF protocol for CHD9 antibody 13402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Carcinog miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.
| ||
Braz J Med Biol Res Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer. | ||
Int J Clin Exp Pathol High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma. | ||
mBio A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.
|