Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 19 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14700 Product name: Recombinant human CHCHD6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC006123 Sequence: MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 6 |
| Calculated molecular weight | 235 aa, 26 kDa |
| Observed molecular weight | 26-29 kDa |
| GenBank accession number | BC006123 |
| Gene Symbol | CHCHD6 |
| Gene ID (NCBI) | 84303 |
| RRID | AB_10697667 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| IHC protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| IP protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| WB protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Bull (Beijing) Mitochondria serve as a source of mineral precursors initiating early cartilage calcification in osteoarthritis. | ||
Mol Cell BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes. | ||
Adv Sci (Weinh) LRRC4 Orchestrates AP2A1-Containing Clathrin-Coated Vesicles to Disrupt Mitochondrial Cristae and Restrict Glioblastoma Progression | ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
| ||
Acta Neuropathol A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease
| ||
J Cell Biol The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy |