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CHCHD2 Monoclonal antibody
CHCHD2 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2G1G10
Cat no : 66302-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, rat liver tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, L02 cells, SMMC-7721 cells, pig liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:1500-1:6000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
Product Information
66302-1-Ig targets CHCHD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CHCHD2 fusion protein Ag24219 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 2 |
| Calculated molecular weight | 151 aa, 16 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC003079 |
| Gene symbol | CHCHD2 |
| Gene ID (NCBI) | 51142 |
| RRID | AB_2881685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHCHD2 antibody 66302-1-Ig | Download protocol |
| IHC protocol for CHCHD2 antibody 66302-1-Ig | Download protocol |
| IF protocol for CHCHD2 antibody 66302-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress | ||
Proc Natl Acad Sci U S A Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms. | ||
Acta Neuropathol Commun Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson's disease. | ||
Hum Mol Genet Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.
| ||
Hum Mol Genet Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.
| ||
Hum Mol Genet CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10.
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