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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CGGBP1 Monoclonal antibody

CGGBP1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F5G9

Cat no : CL488-66524

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66524

    FC experiment of HeLa using CL488-66524

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CGGBP1 (CL488-66524, Clone:2F5G9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CGGBP1 Ready-To-Use IHC Kit. CGGBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66524-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66524 targets CGGBP1 in FC (Intra) applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CGGBP1 fusion protein Ag1129 相同性解析による交差性が予測される生物種
Full Name CGG triplet repeat binding protein 1
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC005222
Gene symbol CGGBP1
Gene ID (NCBI) 8545
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CGGBP1 antibody CL488-66524Download protocol
Standard Protocols
Click here to view our Standard Protocols