Validation Data Gallery

Filter: AllWBIHC

WB analysis using 10716-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10716-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10716-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10716-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10716-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10716-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CGGBP1 Polyclonal antibody

CGGBP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10716-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, chIP, IP-MS, Dot Blot, ELISA

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse thymus tissue, K-562 cells, Raji cells, HL-60 cells, Jurkat cells, rat thymus tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

10716-1-AP targets CGGBP1 in WB, IHC, IF, IP, CoIP, chIP, IP-MS, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1129 Product name: Recombinant human CGGBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC005222 Sequence: MERFVVTAPPARNRSKTALYVTPLDRVTEFGGELHEDGGKLFCTSCNVVLNHVRKSAISDHLKSKTHTKRKAEFEEQNVRKKQRPLTASLQCNSTAQTEKVSVIQDFVKMCLEANIPLEKADHPAVRAFLSRHVKNGGSIPKSDQLRRTYLPDGYENENQLLNSQDC 相同性解析による交差性が予測される生物種
Full Name	CGG triplet repeat binding protein 1
Calculated molecular weight	19 kDa
Observed molecular weight	19-23 kDa
GenBank accession number	BC005222
Gene Symbol	CGGBP1
Gene ID (NCBI)	8545
RRID	AB_2079283
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UFW8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Protocols

Product Specific Protocols
IHC protocol for CGGBP1 antibody 10716-1-AP	Download protocol
WB protocol for CGGBP1 antibody 10716-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IP	Cell Cycle CGGBP1 phosphorylation constitutes a telomere-protection signal. Authors - Umashankar Singh KD Validated View Article
human	chIP	Cell Cycle Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs. Authors - Prasoon Agarwal View Article
human	WB,IP	Epigenetics Chromatin CGGBP1 regulates CTCF occupancy at repeats. Authors - Divyesh Patel View Article
human	IF	Exp Cell Res CGGBP1 is a nuclear and midbody protein regulating abscission. Authors - Singh Umashankar U View Article
human	WB,Dot Blot	BMC Cancer An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1. Authors - Manthan Patel View Article
human	WB	BMC Genet CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity. Authors - Manthan Patel View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10716-1-AP

WB analysis of mouse thymus using 10716-1-AP

WB analysis of K-562 using 10716-1-AP

WB analysis of Raji using 10716-1-AP

WB analysis of HL-60 using 10716-1-AP

IHC staining of human breast cancer using 10716-1-AP

CGGBP1 Polyclonal antibody

CGGBP1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CGGBP1 phosphorylation constitutes a telomere-protection signal.

Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs.

CGGBP1 regulates CTCF occupancy at repeats.

CGGBP1 is a nuclear and midbody protein regulating abscission.

An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.

CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.

Able™

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