Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, human liver tissue, HuH-7 cells |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
15378-1-AP targets CES2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2218 Product name: Recombinant human CES2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 323-623 aa of BC032095 Sequence: LPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL 相同性解析による交差性が予測される生物種 |
| Full Name | carboxylesterase 2 (intestine, liver) |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC032095 |
| Gene Symbol | CES2 |
| Gene ID (NCBI) | 8824 |
| RRID | AB_2077501 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CES2(Carboxylesterase 2) is also named as ICE and belongs to the type-B carboxylesterase/lipase family. Carboxylesterases (CESs) hydrolyze ester groups of drugs and toxins. CES2 specifically catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. CES2 differs from another liver carboxylesterase, CES1 (114835), in drug ester substrate specificity. This protein has 2 isoforms produced by alternative splicing
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CES2 antibody 15378-1-AP | Download protocol |
| WB protocol for CES2 antibody 15378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Metab Dispos Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry. | ||
Mol Metab Carboxylesterase 2 induces mitochondrial dysfunction via disrupting lipid homeostasis in oral squamous cell carcinoma
| ||
Discov Oncol Differential expression of capecitabine-related metabolic enzymes in hepatocellular carcinoma and its clinical value: a retrospective cohort study |
