• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29041-1-AP

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Validation Data Gallery

Applications:
  • WB analysis using 29041-1-AP

    WB analysis using 29041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29041-1-AP

    WB analysis using 29041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 29041-1-AP

    IHC staining of mouse liver using 29041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29041-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CES1 antibody included
  • IF Staining of C2C12 using 29041-1-AP

    IF Staining of C2C12 using 29041-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 29041-1-AP

    IF Staining of HepG2 using 29041-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of C2C12 using 29041-1-AP

    IF Staining of C2C12 using 29041-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inTHP-1 cells, rat liver tissue, hepG2 cells, Caco-2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29041-1-AP targets CES1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES1 fusion protein Ag30534 相同性解析による交差性が予測される生物種
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated molecular weight 566 aa, 62 kDa
Observed molecular weight62 kDa
GenBank accession numberBC012418
Gene symbol CES1
Gene ID (NCBI) 1066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.

Protocols

Product Specific Protocols
WB protocol for CES1 antibody 29041-1-APDownload protocol
IHC protocol for CES1 antibody 29041-1-APDownload protocol
IF protocol for CES1 antibody 29041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols