Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IF | See 14 publications below |
| IP | See 3 publications below |
Product Information
16268-1-AP targets CEP63 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9335 Product name: Recombinant human CEP63 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC014050 Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 63kDa |
| Calculated molecular weight | 703 aa, 81 kDa |
| Observed molecular weight | 57-75 kDa |
| GenBank accession number | BC014050 |
| Gene Symbol | CEP63 |
| Gene ID (NCBI) | 80254 |
| RRID | AB_2077079 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP63 antibody 16268-1-AP | Download protocol |
| IP protocol for CEP63 antibody 16268-1-AP | Download protocol |
| WB protocol for CEP63 antibody 16268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Massive centriole production can occur in the absence of deuterosomes in multiciliated cells. | ||
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Dev Cell A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.
| ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. | ||
