Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
Product Information
28142-1-AP targets CENPE in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CENPE fusion protein Ag27230 相同性解析による交差性が予測される生物種 |
| Full Name | centromere protein E, 312kDa |
| Calculated molecular weight | 316 kDa |
| Observed molecular weight | 300-316 kDa |
| GenBank accession number | NM_001286734 |
| Gene Symbol | CENPE |
| Gene ID (NCBI) | 1062 |
| RRID | AB_2881072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPE antibody 28142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes. | ||
Am J Transl Res Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma. | ||
PLoS One CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival. | ||
Cell Mol Life Sci Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse | ||
Transl Oncol A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity | ||
J Cancer Development and Validation of the novel Cuproptosis- and Immune-related Signature for Predicting Prognosis in Hepatocellular Carcinoma |