• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDKN2A/p19ARF Polyclonal antibody

CDKN2A/p19ARF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28416-1-AP

Synonyms

Arf, ARF INK4a, Cdkn2a, INK4a ARF, Ink4a/Arf, MTS1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF, Pctr1

Validation Data Gallery

Applications:
  • WB analysis using 28416-1-AP

    WB analysis using 28416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28416-1-AP

    WB analysis using 28416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (CDKN2A/p19ARF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28416-1-AP targets CDKN2A/p19ARF in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDKN2A/p19ARF fusion protein Ag28927 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 2A
Calculated molecular weight 18 kDa
Observed molecular weight19 kDa
GenBank accession numberNM_001040654
Gene symbol Cdkn2a
Gene ID (NCBI) 12578
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers.

Protocols

Product Specific Protocols
WB protocol for CDKN2A/p19ARF antibody 28416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols