Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of Jurkat using 11705-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11705-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11705-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 11705-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11705-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11705-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11705-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 11705-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CDK9 antibody (11705-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HeLa using 11705-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (11705-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11705-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK9 Polyclonal antibody (11705-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CDK9 Polyclonal antibody

CDK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 11705-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4, CTK1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human placenta tissue, Jurkat cells, NIH/3T3 cells
Positive IHC detected in	human gliomas tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, U2OS cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

11705-1-AP targets CDK9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2318 Product name: Recombinant human CDK9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-372 aa of BC001968 Sequence: MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF 相同性解析による交差性が予測される生物種
Full Name	cyclin-dependent kinase 9
Calculated molecular weight	372 aa, 43 kDa
Observed molecular weight	38-42 kDa, 55 kDa
GenBank accession number	BC001968
Gene Symbol	CDK9
Gene ID (NCBI)	1025
RRID	AB_2077302
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50750
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Protocols

Product Specific Protocols
IF protocol for CDK9 antibody 11705-1-AP	Download protocol
IHC protocol for CDK9 antibody 11705-1-AP	Download protocol
WB protocol for CDK9 antibody 11705-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP	Cell Inherited blood cancer predisposition through altered transcription elongation Authors - Jiawei Zhao View Article
mouse,human	WB,IP	Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. Authors - Xinzhi Li View Article
mouse	WB	Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) Authors - Xinzhi Li View Article
human	WB	Nat Commun Menin enhances c-Myc-mediated transcription to promote cancer progression. Authors - Gongwei Wu View Article
human	WB	Cell Death Dis CircFUNDC1 interacts with CDK9 to promote mitophagy in nucleus pulposus cells under oxidative stress and ameliorates intervertebral disc degeneration Authors - Tianyuan Gu View Article
human	WB	Proteomics A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand. Authors - Xiaolei Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11705-1-AP

WB analysis using 11705-1-AP

WB analysis of human placenta using 11705-1-AP

IHC staining of human gliomas using 11705-1-AP

IHC staining of human gliomas using 11705-1-AP

IHC staining of human lung cancer using 11705-1-AP

IHC staining of human lung cancer using 11705-1-AP

IF Staining of U2OS using 11705-1-AP

IF Staining of HeLa using 11705-1-AP

FC experiment of HeLa using 11705-1-AP

CDK9 Polyclonal antibody

CDK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inherited blood cancer predisposition through altered transcription elongation

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Menin enhances c-Myc-mediated transcription to promote cancer progression.

CircFUNDC1 interacts with CDK9 to promote mitophagy in nucleus pulposus cells under oxidative stress and ameliorates intervertebral disc degeneration

A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.