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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Mouse CD81 (EAT2)

CD81 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

EAT2

Cat no : CL555-65220

Synonyms

Cd81, CD81 antigen, Tapa 1, Tapa1, Tspan28

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL555-65220

    FC experiment of mouse splenocytes using CL555-65220

    1x10^6 mouse splenocytes were surface stained with 1 ug CoraLite® Plus 555 Anti-Mouse CD81 (EAT2) (CL555-65220, Clone: EAT2) or 1 ug Isotype Control, and 1 ug CoraLite® Plus 647 Anti-Mouse CD45R (B220' target='_blank'>B220) (RA3-6B2) (CL647-65139, Clone: RA3-6B2). Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65220-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL555-65220 targets CD81 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen CD81+ mouse B lymphoma 38C13 相同性解析による交差性が予測される生物種
Full Name CD81 antigen
GenBank accession numberBC011433
Gene symbol Cd81
Gene ID (NCBI) 12520
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD81 antibody CL555-65220Download protocol
Standard Protocols
Click here to view our Standard Protocols