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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

UCHT4

Cat no : CL647-65204

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL647-65204

    FC experiment of human PBMCs using CL647-65204

    1X10^6 human PBMCs were surface co-stained with PacificBlue Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65204, Clone: UCHT4), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65204

    FC experiment of human PBMCs using CL647-65204

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65204, Clone: UCHT4), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65204-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65204 targets CD8 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and Sézary T cells 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
RRIDAB_2883728
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD8 antibody CL647-65204Download protocol
Standard Protocols
Click here to view our Standard Protocols