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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD8 (SK1)

CD8 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

SK1

Cat no : 65146-1-Ig

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32, SK1

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65146-1-Ig

    FC experiment of human PBMCs using 65146-1-Ig

    1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD8 (65146-1-Ig, Clone:SK1) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65146-1-Ig

    FC experiment of human PBMCs using 65146-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8 (65146-1-Ig, Clone:SK1) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65146-1-Ig targets CD8 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
RRIDAB_2918431
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CD8 antibody 65146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols