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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD72 (3F3)

CD72 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

3F3

Cat no : PE-65157

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using PE-65157

    FC experiment of human peripheral blood lymphocytes using PE-65157

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and 20.00 ul/test PE Anti-Human CD72 (PE-65157, Clone:3F3). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using PE-65157

    FC experiment of human peripheral blood lymphocytes using PE-65157

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and PE mouse IgG2b isotype control antibody. Cells were not fixed.

Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65157 targets CD72 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Normal human lymphocytes from a lymph node 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
GenBank accession numberBC011734
Gene symbol CD72
Gene ID (NCBI) 971
RRIDAB_2883935
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
FC protocol for PE CD72 antibody PE-65157Download protocol
Standard Protocols
Click here to view our Standard Protocols