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- KD/KO Validated
CD71 Monoclonal antibody
CD71 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IP, IHC, IF, FC, Blocking assay, ELISA
Conjugate
Unconjugated
CloneNo.
3C11F11
Cat no : 66180-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-251 cells, Raji cells, A549 cells, TF-1 cells, HeLa cells, U2OS cells, A431 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue, MCF-7 cells |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
66180-1-Ig targets CD71 in WB, IP, IHC, IF, FC, Blocking assay, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD71 fusion protein Ag21612 相同性解析による交差性が予測される生物種 |
| Full Name | transferrin receptor (p90, CD71) |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC001188 |
| Gene symbol | TFRC |
| Gene ID (NCBI) | 7037 |
| RRID | AB_2881575 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD71 antibody 66180-1-Ig | Download protocol |
| IHC protocol for CD71 antibody 66180-1-Ig | Download protocol |
| IF protocol for CD71 antibody 66180-1-Ig | Download protocol |
| IP protocol for CD71 antibody 66180-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chem Eng J Functionalized biological metal–organic framework with nanosized coronal structure and hierarchical wrapping pattern for enhanced targeting therapy | ||
Cell Death Dis Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma | ||
Free Radic Biol Med Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity | ||
Bioeng Transl Med Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy. | ||
PLoS Genet SNX-3 mediates retromer-independent tubular endosomal recycling by opposing EEA-1-facilitated trafficking. | ||
J Biol Chem Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation. |
