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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

H1.2F3

Cat no : FITC-65105

Synonyms

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using FITC-65105

    FC experiment of mouse splenocytes using FITC-65105

    1X10^6 anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD69 (FITC-65105, Clone:H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug FITC Anti-Mouse CD69 (FITC-65105, Clone:H1.2F3). Cells were not fixed.

Tested Applications

Positive FC detected inAnti-CD3/CD28-treated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Mouse dendritic epidermal T cell line Y245 相同性解析による交差性が予測される生物種
Full Name CD69 antigen
GenBank accession numberBC106997
Gene symbol Cd69
Gene ID (NCBI) 12515
RRIDAB_2883777
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD69 antibody FITC-65105Download protocol
Standard Protocols
Click here to view our Standard Protocols