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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H1.2F3

Cat no : CL647-65105

Synonyms

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

Validation Data Gallery

Applications:
  • FC experiment of BALB/C mouse splenocytes using CL647-65105

    FC experiment of BALB/C mouse splenocytes using CL647-65105

    1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface co-stained with 0.5 ug FITC Plus Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD69 (CL647-65105, Clone: H1.2F3). Cells were not fixed.

Tested Applications

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65105-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Mouse dendritic epidermal T cell line Y245 相同性解析による交差性が予測される生物種
Full Name CD69 antigen
GenBank accession numberBC106997
Gene symbol Cd69
Gene ID (NCBI) 12515
RRIDAB_2883683
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD69 antibody CL647-65105Download protocol
Standard Protocols
Click here to view our Standard Protocols