CoraLite® Plus 647 Anti-Mouse CD62L (L-Selectin) (MEL-14)
CD62L (L-Selectin) Monoclonal Antibody for FC, IF
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
IF, FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
MEL-14
Cat no : CL647-65123
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL647-65123 targets CD62L (L-Selectin) in IF, FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | C3H/eb mouse B lymphoma 38C-13 相同性解析による交差性が予測される生物種 |
| Full Name | selectin, lymphocyte |
| GenBank accession number | BC052681 |
| Gene symbol | Sell |
| Gene ID (NCBI) | 20343 |
| RRID | AB_2883696 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CD62L (L-Selectin) antibody CL647-65123 | Download protocol |
| FC protocol for CL Plus 647 CD62L (L-Selectin) antibody CL647-65123 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
