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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Mouse CD54 (YN1/1.7.4)

CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

YN1/1.7.4

Cat no : PE-65066

Synonyms

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using PE-65066

    FC experiment of mouse splenocytes using PE-65066

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug PE-Anti-Mouse CD54 (PE-65066, clone YN1/1.7.4) (red) or 0.20 ug PE-rat IgG2b isotype control (blue). Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65066 targets CD54 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse NS-1 cells 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
GenBank accession numberBC008626
Gene symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2883863
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for PE CD54 antibody PE-65066Download protocol
Standard Protocols
Click here to view our Standard Protocols