• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD54 (YN1/1.7.4)

CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

YN1/1.7.4

Cat no : 65066-1-Ig

Synonyms

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using 65066-1-Ig

    FC experiment of mouse splenocytes using 65066-1-Ig

    1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD54 (65066-1-Ig, Clone: YN1/1.7.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black). Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65066-1-Ig targets CD54 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse NS-1 cells 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
GenBank accession numberBC008626
Gene symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2918373
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for CD54 antibody 65066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols