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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65150-1-Ig

Synonyms

B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

Validation Data Gallery

Applications:
  • IF Staining of human colon cancer using 65150-1-Ig

    IF Staining of human colon cancer using 65150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of human PBMCs using 65150-1-Ig

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65150-1-Ig

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive IF detected inhuman colon cancer tissue
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65150-1-Ig targets CD45RO in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
RRIDAB_2881476
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IF protocol for CD45RO antibody 65150-1-IgDownload protocol
FC protocol for CD45RO antibody 65150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols