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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Rat CD45RA (OX-33)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

OX-33

Cat no : CL555-65234

Synonyms

CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200

Validation Data Gallery

Applications:
  • FC experiment of rat splenocytes using CL555-65234

    FC experiment of rat splenocytes using CL555-65234

    1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Rat CD45RA (CL555-65234, Clone: OX-33) or 0.5 ug CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21) (CL555-65124, Clone: MOPC-21). Cells were not fixed.

  • FC experiment of rat splenocytes using CL555-65234

    FC experiment of rat splenocytes using CL555-65234

    1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Rat CD45RA (CL555-65234, Clone:OX-33) (red), or 0.5 ug CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21) (CL555-65124, Clone: MOPC-21) (blue). Cells were not fixed.

Tested Applications

Positive FC detected inrat splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65234-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL555-65234 targets CD45RA in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Leukocyte common antigen purified from rat splenocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight143 kDa
GenBank accession numberXM_006249910
Gene symbol Ptprc
Gene ID (NCBI) 24699
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification Method N/A
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD45RA antibody CL555-65234Download protocol
Standard Protocols
Click here to view our Standard Protocols